Descrição
Overview
Standard curve
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 10.00 | 1.961 | 1.875 |
| 5.00 | 1.587 | 1.501 |
| 2.50 | 1.185 | 1.099 |
| 1.25 | 0.869 | 0.783 |
| 0.63 | 0.549 | 0.463 |
| 0.32 | 0.366 | 0.280 |
| 0.16 | 0.179 | 0.093 |
| 0.00 | 0.086 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant NFκB-p65 and the recovery rates were calculated by comparing the measured value to the expected amount of NFκB-p65 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 80-92% | 86% |
| EDTA plasma(n=5) | 83-97% | 90% |
| Heparin plasma(n=5) | 86-99% | 92% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NFκB-p65 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 86-93% | 93-101% | 85-98% | 90-101% |
| EDTA plasma(n=5) | 89-99% | 83-99% | 98-105% | 96-101% |
| Heparin plasma(n=5) | 85-92% | 79-96% | 89-101% | 85-97% |
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